CombiFlash® EZ Prep

Streamline Flash and Preparative HPLC chromatography with a compact, easy-to-operate unit 

The CombiFlash® EZ Prep offers streamlined Flash and Prep HPLC chromatography in one compact, easy-to-operate unit. With just two clicks, users can switch from pre-purifying samples on Flash to generating submission-quality compounds on Prep ​HPLC. Teledyne Isco's RFID-encoded collection racks ensure proper loading every time and allows users to hot-swap racks during separation for virtually limitless fraction collection capability.​

The EZ Prep also comes equipped with Teledyne Isco's PeakTrak® software, a comprehensive package which integrates all detector options for single-point control. Designed with simplicity and ease-of-use in mind, PeakTrak allows even the most novice users to begin purifying samples within minutes.

Standard features:

  • Up to 3500 psi (240 bar) and 200 mL/min
  • Run Prep ​HPLC columns up to 50 mm in diameter (including 5 µm particle diameter)
  • Flash purification for 10 mg to 33 g followed by final compound purification on high-performance columns
  • UV, UV-Vis, ELSD, and MS detection options available
  • Switch between normal and reverse phase solvents

 

CombiFlash® EZ Prep Specifications

Flow Rate Range​

5 - 200 mL/min (Flash & Prep)​

​Pump Prime ​Self-priming
​​Maximum Pressure (Flash) ​​200 psi (13.8 BAR)
​Maximum Pressure (Prep) ​​3,500 psi (240 BAR)
​​UV and UV-Vis Detection ​​UV 200-400 nm (standard); UV-Vis 200-800 nm (optional)
​​ELSD Detection ​​Built-in ELSD detector compatible with UV or UV-Vis
​Mass Spectrometer ​Three models with mass ranges of 10-1,200 and 10-2,000 Da
​Number of Solvents ​Binary gradient between any two of four solvents
​Programmable gradients ​Linear, step, isocratic
​Sample introduction (Flash) ​Solid or liquid load
​Sample introduction (Prep) ​Variable volume liquid load (5mL loop standard)
​User Interface ​10.4 in touchscreen
​Fraction Collector ​Internal
​Dimensions ​ ​​​26.8" x 16.9" x 17" (68 x 43 x 43.2 cm)

 

​For a full list of product specifications, see the datasheet.

Download Datasheet

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CombiFlash® Rf+

RFID method automation, more fractions, larger screen, higher pressure – all in a smaller package. 

CombiFlash® Rf+The CombiFlash® Rf+ improves your productivity whether you purify synthetic compounds, natural products, peptides, or polymers. With operating pressures up to 200 psi (13.8 bar) the CombiFlash Rf+ is compatible with both normal and reversed phase purification conditions. 

The TLC to Gradient calculator automatically determines optimal separation parameters while minimizing run time. 

There is no need to wait to access data - built in networking allows you to connect to the CombiFlash® Rf+ to monitor and remotely control your experiment, analyze data, and print to network printers. 

View UV spectra in real time - during peak elution. Review the spectra to confirm that the system is monitoring the correct wavelength. Interactive screen lets you change parameters instantly during the run if another wavelength would be more appropriate. 

Rapid column air purge prevents solvent from being discarded with the used column. It is better for the environment, safer in the lab, and eliminates messy column removal. 


Easy scale-up with built-in method-scaling means you won't need to re-develop your method. You save time when you increase reaction size. You can even scale up to a 3 kg column run on a CombiFlash Torrent.

Standard Features

  • One simple push of a button and your purification is underway. 
  • UV Spectra display in real time and post run. 
  • Compact design saves valuable bench or hood space. 
  • RFID (Radio Frequency Identification) technology automates setting the parameters for purification runs and fraction collection. 
  • Built-in solvent and waste management prevents columns from drying, and messy waste overfills. 
  • Large 10.4" (26.4cm) touch screen provides a simple to use and informative user interface through our intuitive PeakTrak software.

CombiFlash® Rf+

​Flow Rate Range ​1 - 200 mL/min
​Pressure Limit ​200 psi (13.8 bar)
​UV and UV- Vis Detection ​Variable UV 200 - 400 nm standard. Variable UV-Vis 200 - 800 nm option
Number of Solvents Binary gradient with any two of four solvents; a third solvent can be used to add modifier at a fixed percentage
Programmable Gradients Linear, step, isocratic
​Sample Injections Auto-inject with 6-position automated motorized sample loading valve;self-cleans at end of run; solid or liquid injection
User Interface 10.4" touchscreen
​Fraction Collector Internal
​Dimensions (H x W x D) ​24 x 14.1 x 17 in​ (61.0 x 35.8 x 43.2 cm)
​Weight  ​54 lbs (24.5 kg)

 

Download Datasheet

CombiFlash® Rf+ PurIon

Verify before you purify 

The CombiFlash Rf+ PurIon takes the guesswork out of your purification routine with mass directed purification. Identify your peak of interest at the time of elution with the advantage of targeted fraction collection. Simply instruct the Flash system to collect only those peaks matching the mass of your target compound, eliminating the need for further analysis and saving time in post-purification processing. 

As with all other CombiFlash models, Teledyne Isco's intuitive PeakTrak® software lets you access all the commands you need from a single touchscreen. The method development screen facilitates confirmation of the detectable ions of your product, and helps you identify and set appropriate detection parameters prior to the purification process.

Standard Features

  • Real-time method editing and control 
  • Touch-and-drag gradients 
  • Real-time and post-run MS and UV spectral display 
  • Flow rates to 200 mL/min at pressures up to 200 psi (13.8 bar) 
  • Compatible with column sizes from 4 to 330 grams 
  • Mass range from 50 to 1200 Dalton with 1 Dalton resolution 
  • Use with your choice of ELSD, UV, or UV- Vis detectors​

​CombiFlash® Rf+ PurIon

Flow Rate Range

​1 - 200 mL/min

​Pressure Limit

200 psi (13.8 bar)

​UV and UV- Vis Detection

​Variable UV 200 - 400 nm standard. Variable UV-Vis 200 - 800 nm option

​ELSD Detection

​Option that can be combined with either UV or UV-Vis

​Mass Spectrometry Detection

​50 – 1200 Dalton, 1 Dalton Resolution 

Electrospray Ionization (ESI) or optional Atmospheric Pressure Chemical Ionization (APCI)

 

Positive or Negative Ionization Mode

​SIM Sensitivity

​10 pg Reserpine, 100:1 S/N (RMS) with SIM of m/z

​Number of Solvents

​Binary gradient with any two of four solvents; A third solvent can be used to add modifier at a fixed percentage.​

​Programmable Gradients

​Linear, step, isocratic

​Sample Injection

​Direct

​User Interface

​10.4" touchscreen

​Fraction Collector

​Internal

​Dimensions (H x W x D)

​CombiFlash® Rf - 24 x 14 x 17 in (60 x 36 x 43 cm);

Mass spectrometer - 26 x 11 x 22 in (66 x 28 x 56 cm);

Roughing pump - 10 x 9 x 18 in​ (26 x 23 x 46 cm)​

For a full list of product specifications, see the datasheet.

Download Datasheet

CombiFlash® Rf+ Lumen

The smallest Flash system with integrated UV, UV-Vis, and ELS detection 

The CombiFlash Rf+ Lumen system enhances detection capabilities with an integrated evaporative light scattering detector (ELSD). In addition to UV or UV-Vis detection for absorbing compounds, the ELSD detects compounds with little or no chromophores such as carbohydrates, steroids, and lipids. The integrated ELSD conserves valuable bench space. 


Large fraction capacity allows longer runs without changing racks. You don't need to divert time from other activities to watch your purification. An automated injection valve lets you load samples and walk away - and it's self-cleaning to wash away materials that can contaminate the next run. 

Whether you separate synthetic compounds, natural products, peptides, polymers, or other chemical separations; the CombiFlash Rf+ Lumen improves your compound's detectability. 


The ELSD withdraws a miniscule portion of the liquid eluting from the chromatographic column into the detector module. This fluid is then nebulized using a stream of inert gas. This mixture forms an aerosol cloud of solvent droplets containing your compound. The aerosol cloud is then evaporated, leaving micro-particles of dried compound. 

The gas stream carries these micro-particles to the detector where a laser light illuminates the gas stream. When a compound is present, the particles cause the laser light to scatter, indicating its presence and creating a detector signal. This signal triggers the fraction collector to collect your compound.

Standard Features

  • Space saving design is smaller than other flash chromatography systems on the market, freeing scarce hood space for other uses. 

  • Solvent and waste management prevents columns from drying and waste containers from hazardous overflows. 

  • Large touch screen 10.4 inch (26.4 cm) puts the most used controls on a single, easy to read display. 

  • RFID (Radio Frequency Identification) identifies columns and loads the appropriate method for the best separation. With reusable bonded-phase columns, the system tracks the number of runs on the column as well as the last solvent used, so you know whether you need to flush the column. 

  • RFID identification of fraction collection racks sets the maximum fraction size to prevent overflows. 

  • No waiting to access data - networking lets you connect to the CombiFlash Rf+ to monitor and remotely control your experiment, analyze data, and print to network printers. 

  • Rapid column air purge prevents solvent from being discarded with the used column. It is better for the environment, safer in the lab, and eliminates messy column removal. 

  • TLC Rf to Gradient calculator determines optimal separation parameters while minimizing run time. 

  • Easy scale-up with built-in method-scaling means you won’t need to re-develop your method. You save time when you increase reaction size. You can even scale up to a 3 kg column run on a CombiFlash Torrent. 

  • Back pressures to 200 psi for improved separations. 

  • May be ordered in either variable UV 200 - 400 nm, or variable UV-Vis 200 - 800 nm configurations

 

CombiFlash® Rf+ Lumen

​Flow Rate Range

​1 - 200 mL/min

​Maximum Pressure

​200 psi (14 bar)

​Detection

​UV (UV-Vis optional) + ELSD

​Spray Chamber Temperature

​Ambient to 120 °C

​Drift Tube Temperature

​10 - 70 °C

​Gas Requirements

​65 psi (4.6 BAR) ± 5 psi (0.3 BAR) Nitrogen or other inert gas

​​Dimensions (H x W x D)

​​24 x 14.1 x 17 in (61.0 x 35.8 x 43.2 cm)

 

Download Datasheet

CombiFlash® Rf 150

Begin purifying samples in minutes 

The CombiFlash ​​Rf 150 delivers reversed phase purifications at a fraction of the cost of preparative HPLC. The system operates at pressures up to 150 psi (10.3 bar) for your separation needs. The rugged construction you have come to expect from Teledyne Isco makes this a daily workhorse for your demanding routine. 

The CombiFlash Rf 150 is designed to exceed expectations in both performance and reliability. Driven through our highly acclaimed PeakTrak user interface, even a beginning user will have this system up and running in a matter of minutes. Don't waste your valuable time learning complex software when you can begin purifying samples within minutes. As with all other CombiFlash models, Teledyne Isco's intuitive PeakTrak software lets you access all the commands you need from a single touchscreen. 

Quickly program binary linear gradients, define a custom step gradient, or set isocratic purification conditions using our on board graphical interface, then monitor the purification remotely from your office computer or iPad. 

The system accepts a variety of rack sizes so that samples can be collected in volumes appropriate for the column being used. 

Our wide flow rate range supports the use of column sizes from 4 grams to 330 grams of packing.

Standard Features

  • Real-time method editing and control 

  • Touch-and-drag gradients 

  • TLC to gradient optimization 

  • Flow Rates to 100 mL/minute for fast purifications 

  • Pressures up to 150 psi (10.3 bar) 

  • Variable wavelength UV detector

CombiFlash® Rf 150

​Flow Rate Range

​10 - 100 mL/min

​Pressure Limit

​150 psi (10.3 BAR)

​Column Sizes

​4 - 330 g

​Detection

​Variable UV 200 - 400 nm standard; 

Variable UV-Vis 200 - 800 nm optional

​User Interface

​10.4 in touchscreen (26.4 cm)

​Dimensions (H x W x D)

​24 x 14.1 x 17 in (60 x 36 x 43 cm)

 

Download Datasheet

CombiFlash Torrent®

The next generation in scale up Flash purification

Get the ease of​ use and reliability provided by CombiFlash and RediSep for Development Scale purification with the CombiFlash Torrent Purification System from Teledyne Isco. One liter per minute flow rates rapidly purify up to 300 grams of sample. The 100 psi pumps accurately deliver a reproducible binary gradient. The CombiFlash Torrent system is designed to be flexible for a variety of large-scale purification needs, with various methods of sample introduction and collection. 

The trusted RediSep columns communicate with the system to quickly create methods without error through RFID technology. Column information is accessed on the RediSep column for easy recall of methods. Large scale RediSep columns, with quick Luer connections, are available in a broad range of sizes and medias.

Standard Features

  • 1 L/min flow rate 
  • 100 psi rated 
  • 300 g purifications 
  • Patented solvent and waste level sensing technology 
  • Fully-grounded fluid path 
  • Easy-to-use PeakTrak software 
  • Accepts RediSep column sizes from 80 g to 3 kg 
  • RFID column detection 
  • Automated six-port valve and large diameter tubing​​

CombiFlash Torrent

​Flow Rate Range ​50 - 1000 mL/min
​Pressure Limit ​100 psi (6.89 BAR)
​Sample Size ​0.5 - 300 g
​Column Sizes ​80 g - 3 kg
​​Dimensions (H x W x D) ​​28.1 x 18.5 x 17.9 in​ (71.4 x 47 x 43.5 cm)​
​​Weight ​​65 lbs (29.5 kg)​​

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