New PTI QuantaMaster 8000 Series Fluorometers

Overview

New PTI QuantaMaster™ 8000 Series Fluorometers

Two years after the acquisition of Photon Technology International (PTI), HORIBA is proud to introduce the new PTI QuantaMaster™ 8000 series of fluorometers. The new PTI QuantaMaster 8000 series modular research fluorometers from HORIBA Scientific offer the world’s highest guaranteed sensitivity specification, plus many unique benefits. Only the HORIBA Fluorolog 3, also from HORIBA Scientific, matches the sensitivity of the new QuantaMaster 8000 series.

  • Highest guaranteed sensitivity specification at 30,000:1 Water Raman RMS
  • World class software for all steady state and lifetimes needs
  • Extended wavelength range with triple grating monochromators
  • Ultimate flexibility with up to four light sources and six detectors that can be selected
  • Fully automated system
  • Best in class stray light rejection with large single or double additive, coma corrected, monochromators
  • World class TCSPC lifetime enhancements
  • NIR steady state and phosphorescence lifetime detection to 5,500 nm
PTI QuantaMaster Fluorometer
Water Raman spectrum of the PTI QuantaMaster, resulting in a signal to noise ratio of 30,000:1! Experimental conditions: λex = 350 nm, Δλex = Δλem = 5 nm, int = 1 s.

Ultimate Sensitivity

The industry standard for sensitivity is a signal to noise ratio calculated from a water Raman spectrum. Using this standardized test (see our technical note "SN Determination for PTI QuantaMaster Fluorometer,") our signal to noise ratio is the highest sensitivity in the world, matched only by the Fluorolog3, also from HORIBA. The extreme sensitivity of the PTI QuantaMaster fluorometer is even more remarkable because it is achieved with the lowest wattage lamp in the industry. This remarkable achievement is a result of the intelligent engineering of the unique PowerArc™ arc lamp illuminator featuring an ellipsoidal reflector with the highest possible light gathering efficiency of 67%, and focusing the light in a tight spot at the monochromator slit. As a result, the standard 75W Xe delivers light to the sample more efficiently than higher power lamps featured by other instruments. This reduces energy waste and excessive heat generation by an overpowered light source, not to mention cost, while exceeding the sensitivity of all competitors' designs. Another contribution to the high sensitivity of the PTI QuantaMaster comes from the asymmetrical, aberration-corrected monochromator, optimized for the best light throughput and stray light rejection.

 
PTI QuantaMaster 75W Xe lamp
Superior optical design of the PTI QuantaMaster 75W Xe lamp illuminator is a key factor in PTI QuantaMaster sensitivity

Want a higher wattage light source?

No problem. We also offer a 450W xenon illuminator. It is just a little more expensive. Unfortunately however it does not offer any increase in sensitivity, because it is not taking advantage of the ultimate collection efficiency of the PowerArc illuminator. 1.49 mln cts/s, SN=30,000:1 

Ultimate Stray Light Rejection

PTI ultimate Stray Light Rejection
Raman spectrum of CCl4 using PTI QuantaMaster with single excitation & single emission monochromators. Well-resolved peaks and no contamination from the Rayleigh scattering demonstrates the excellent stray light suppression. Experimental conditions: λex = 349 nm, Δλex = 0.7 nm, Δλem = 0.7 nm, step size=0.05 nm, integration time = 1 s

With Single or Optional Double Additive Monochromators

Suppression of stray light is one of the most critical factors when measuring highly scattering, or low quantum yield samples. Every PTI QuantaMaster series spectrofluorometer is custom made with the highest quality optics to insure the lowest amount of scatter. The standard 300 mm focal length asymmetrical Czerny-Turner monochromators are coma-corrected and individually optimized for purpose as either excitation or emission monochromators, ensuring the lowest amount of stray light contamination for the best detection of the true fluorescence signal. These monochromators boast an impressively high stray light rejection of 1x10-5 in a single excitation monochromator configuration. The excitation and emission monochromators in the QuantaMaster can also by upgraded to double additive 300 mm focal length monochromators, improving the stray light rejection to 1x10-10. The QuantaMaster also offers an optional order sorting filter wheel for rejection of second order signals from large spectral scans. This ultimate stray light performance was motivated by an increasing demand for photoluminescence spectrometers in materials science, where strongly scattering samples, such as powders, wafers and films, are routinely used. Very low stray light performance will also benefit researchers working in biological, biomedical and environmental areas where cell suspensions, protein and biomembrane solutions, or soil samples, generate high levels of scattered light.

 

Resolution

PTI QuantaMaster spectrofluorometers
Emission scan of dysprosium-doped YAG crystal measured at 78K in software controlled LN cryostat illustrating excellent resolution of narrow spectral lines attained at low temperature.

Resolution is of utmost importance to photoluminescence research. High quality resolution can reveal detailed spectral features which are indispensable for applications in materials science and analytical chemistry. Resolution is the key to detecting very narrow lines, which is necessary to study fine interactions in inorganic materials and crystals. The PTI QuantaMaster yields high quality resolution due to innovative optical design, and very minimal optical aberrations.

The PTI QuantaMaster spectrofluorometers use a precision-driven asymmetrical, 300 mm focal length Czerny-Turner monochromator with a triple motorized grating turret and motorized flipping mirrors. More than 30 different gratings are available. Due to the combination of the computer-controlled motor with micro-stepping resolution and available grating selection, it is possible to achieve a minimum 0.022 nm step size. This means that within the UV and VIS spectral regions, you can resolve spectral features well below 0.1 nm.

PTI QuantaMaster System Specifications*

PTI QuantaMaster 8000
Series

PTI QuantaMaster 300 Series

PTI QuantaMaster 800

Signal to Noise Ratio

30,000:1 or better

300: 1500:1 or better
300 Plus: 3000:1 or better

6000:1 or better

Data Acquisition Rate

1,000,000 points/sec. to
1 point/1000 sec

300/300 Plus: 1 point/sec to
300 points/sec
310: Up to 20 points/sec

1,000,000 points/sec.
to 1 point/1000 sec

Inputs

4 photon counting (TTL); 4 analog (+/- 10 volts) ; 1 analog reference channel (+/- 10 volts); 2 TTL

Outputs

2 analog (+/- 10 volts) 2 TTL

Emission Range

185 nm to 900 nm
(optional to 5,500 nm)

185 to 680 nm
(optional to 5,500 nm)

185 to 680 nm
(optional to 1,010 nm)

Light Source

High efficiency “ECO” friendly
continuous 75 W Xenon arc
lamp (Optional 450 W xenon)

300/300 plus: High power
Xenon flash lamp
310: Nitrogen laser pumped
dye laser

High efficiency “ECO” friendly
continuous 75 W Xenon arc
lamp

Monochromator

300 mm, coma-aberration corrected, asymmetrical, excitation or emission optimized, Czerny-Turner design

Focal Length

300 mm

300 mm

150 mm excitation
300 mm emission

Slits

Computer controlled,
continuously adjustable

Manual or computer
controlled, continuously
adjustable

Manual continues Excitation,
Manual or computer controlled
continuous Emission

Emission Grating

1200 line/mm 400 nm blaze
(Plus two optional gratings)

1200 line/mm 400 nm blaze
(Plus two optional gratings)

1200 line/mm 400 nm blaze
(Plus two optional gratings)

Optional Grating

 

75 to 2400 line/mm and holographic models available

Wavelength
Accuracy

+/- 0.3 nm

+/- 0.3 nm

+/- 0.05 nm excitation
+/- 0.3 nm emission

Minimum Step Size

0.022 nm

0.022 nm

0.05 nm excitation
0.022 nm emission

Lifetime Range

N/A

1 μs to seconds

N/A

Detection

Multimode: Photon Counting, 3 analog (fast, medium, slow response), direct and Single-Shot
Transient Digitizer (SSTD) mode

System Control

Computer interface with spectroscopy software

*Components, dimensions and specifications vary depending on the specific QuantaMaster configuration and accessories selected.

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Download Datasheet

 

Fluorolog®

 

Description

The Fluorolog®-3 is a unique, modular system which allows the researcher to interchange a versatile range of accessories to correspond perfectly with the characteristics of a given sample. From analysis of steady-state or molecular dynamics to IR probes, the Fluorolog®-3 comes equipped with a wide range and limitless configuration of accessories to enhance the accuracy and speed of your application.

Features

  • World's most sensitive spectrofluorometer *
  • Can detect 50-femtomolar fluorescein
  • Unique, modular system adapts to new experiments or changing technology so is never obsolete
  • Interchange wide range of computer-controlled accessories: spectrometers, detectors, sources, and more!
  • Totally computer-controlled
  • All-reflective optics so that the sample is in focus at all wavelengths
  • Time-correlated single-photon counting (TCSPC) accessory for lifetime determinations
  • Phosphorimeter accessory for long-lived luminescence determinations

FluorEssence Steady State and Frequency Domain Software

FluorEssence - Steady State and Frequency Domain Software

FluorEssence™ for Windows® sets a new standard for fluorescence software. FluorEssence™ merges spectrofluorometer data-acquisition directly to the famous processing power of Origin® software.

Simple clicks let you select a scan type, accessory or bring back a complete experimental set-up.

Revolutionize the Way You Operate Your Spectrofluorometer:

  • Simplified windows make data-acquisition intuitive even to the casual user.
  • With detector algebra, assemble input signals from different detectors into unique equations (including arithmetic or transcendental functions) that produce data in a format tailored to your application.
  • Select the special Real Time Control (RTC) window to optimize your run parameters before actually scanning.
  • Free video tutorials make FluorEssence™ even easier to learn.
  • Run a variety of accessories including polarizers, microwell-plate readers, temperature baths, autotitrators, phosphorimeters, and more.
  • Automatic correction for blanks and lamp output.
  • Change units for temperature, wavelength, and slit-width with the click of a mouse.

FluorEssence™ also includes the powerful processing and data-management tools of Origin® to streamline your laboratory’s operation.

Data-viewing, plotting, processing and management are all available, including extensive import and export facilities, view manipulation and creation, arithmetic applications, peak-picking and integration, data-management and much more!

 

Software Features

  • Data views in Workbook formats, keeping graphs, tables and notes together for each experiment
  • Zooming and scaling
  • Contour maps and profiles from 3D plots
  • Integration/DerivativeFit fluorescence data to Gaussian, Lorentzian, and custom curves
  • Standard arithmetic
  • 3D perspective
  • Smoothing
  • Deconvolute
  • Excitation/emission correction
  • Interpolation and extrapolation
  • Blank subtraction
  • Normalization
  • PLQY calculator wizard (for use with Quanta-Phi accessory)

 

DAS6 Decay Analysis Software

HORIBA Scientific decay analysis software recovers kinetic information (such as fluorescence lifetime and rotational correlation times) from luminescence decay data by fitting the raw decay data to one of a selection of kinetic models. Features such as shift iteration and reconvolution of instrument response function (IRF, or "lamp" profile) allow accurate recovery of multiple decay components even when the decay data is grossly distorted by the IRF.

DAS6 is designed to streamline the analysis of time-domain luminescence data, while still allowing for fine-tuning of parameters where necessary.  Analysis modules include reconvolution analysis of multi-exponential models plus analysis of more specialised fluorescence decay processes as detailed below.

Multi-Exponential

Analysis for up to 5 exponentials.

Batch Exponential

Batch mode supports datasets containing up to 10,000 decays and is ideal for applications where large amounts of lifetime data are generated e.g. lifetime imaging and reaction monitoring.  It features full reconvolution with up to five exponentials and shift iteration.

Global Exponential

Perform global analyses of up to 5 exponential components and 100 decay curves.

Distribution

Two methods are available for performing lifetime distribution analysis.  The first method employs a “Top Hat” distribution function which allows a single lifetime distribution to be fitted in conjunction with an additional discrete lifetime.  The second method is an optional module which will perform a Non-Extensive Decay distribution which allows up to five lifetime distributions to be modelled.

Exponential Series

Exponential series analysis of up to 30 terms.  This allows parameterisation of otherwise difficult data.

Foerster Energy Transfer

2D and 3D Foerster type energy transfer with an optional exponential component.

Yokoto-Tanimoto Energy Transfer

Yokoto-Tanimoto energy transfer taking into account diffusion with an optional exponential component.

Micellar quenching

Micellar quenching kinetics can help determine the surfactant aggregation number.

Exciplex

Exciplex analysis follows formation of an excited state species.

Anisotropy analysis

Impulse reconvolution of up to 2 correlation times and 5 fluorescence decay components.  Each model parameter, including shift, can be fixed at a pre-determined value or optimised as part of the parameter fitting.  The use of reconvolution allows accurate determination of decay times and rotational correlation times even when the observed decay is grossly distorted by the duration of the excitation pulse.

DataStation TCSPC Acquisition Software

DataStation is the latest measurement environment for time-domain fluorescence and is supplied with all HORIBA Scientific lifetime systems.

Lifetime systems are made up of various modules and components, including pulsed light sources, PMTs, monochromators, motorized polarizers, focusing optics, MCAs etc. DataStation integrates the control of all these components into one intuitive, mouse-driven Windows environment.

Lifetime systems can perform a variety of measurement functions, such as fluorescence decay measurement, phosphorescence decay measurement, time-resolved anisotropy measurements, steady-state spectra, time-resolved emission scan (TRES), temperature scans and more. These measurements are now routine using DataStation. Measurement templates are provided to quickly set up the instrument for the required measurement, and automated data acquisition is performed using computer controlled components.

Acquired data can be saved in a variety of formats including the .das format, HORIBA Jobin Yvon IBH v4.2, and ASCII, printed to your Windows printer, exported or copied to the clipboard for manipulation using a third-party application.

DataStation can be purchased separately for use on non-HORIBA Scientific systems, and we are happy to add custom functionality and interfacing (e.g. to additional monochromator types, motorized polarizer holders etc).

The DataStation software comes into its own when combined with a FluoroHub.

Functionality

DataStation evolves continually as new functionality is added in response to customer suggestion. The following is a partial list:

Graphical

  • Log or linear y-axis.
  • Auto scaling y-axis.
  • Multiple mouse zoom modes - x only, y only, x and y, pan, reset.
  • Reversed time-axis mode (automatically reverses data during reversed-TAC mode measurements).
  • Live display of cursor position, peak count, peak channel and total count.
  • Detachable and customizable toolbars with helpful hints.
  • Context-sensitive pop-up toolbars.
  • Intuitive tree navigation for files and hardware settings.
  • Tree navigation control window for FluoroHub control.
  • Real-time estimated average lifetime during acquisition.

TCSPC mode

  • Measurement presets - peak count or elapsed time.
  • Multiple traces on screen simultaneously.

TRES mode

  • Configure instrument for scan operation in just a few mouse clicks.
  • Scan excitation or emission monochromator.
  • Records 3D data (counts vs time vs wavelength) with live 3D progress display and interactive rotation.
  • Manual and automatic data slicing.

Anisotropy mode

  • Target difference count.
  • G-factor measurement.

MCA support

  • Canberra/Tennelec PCA3.
  • EG&G/Perkin Elmer Ortec Trump card.

Monochromator support

Polarizer support

  • HORIBA Scientific 5000U-02 polarizers.
  • PRA polarizers.

Temperature control support

  • HORIBA Jobin Yvon IBH dipstick temperature controller (as accessory to HORIBA Scientific 5000 series system only).
  • Cryostat (as accessory to HORIBA Scientific 5000 series system only).

System Requirements

The following PC specification are required to run DataStation. Measurement functionality depends on spectrometer hardware attached. Basic operation requires a supported MCA card or FluoroHub. Contact HORIBA Scientific for more information.

1 Gb RAM, 1 Gb HD space, DVD drive, 1024x768 graphics with minimum 256 color support, mouse or other pointing device. Windows™ XP or later (XP/Vista/7). Windows™ XP recommended.

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FluoroMax Series

 

Features

  • Water Raman signal-to-noise ratio: 16000:1 (6000:1 FSD)
  • Photon Counting for Ultimate Sensitivity
  • Fast Scanning capability - up to 80 nm/second
  • Powerful FluorEssence™ software for Windows®
  • Method Files to recall complete experiment parameters
  • Time-correlated single-photon counting (TCSPC) accessory for lifetime determinations
  • Phosphorimeter accessory for long-lived luminescence determinations

 

Hardware

The Source

The xenon source that supplies prime UV performance is focused onto the entrance slit of the excitation monochromator with an elliptical mirror. Besides ensuring efficient collection, the reflective surface keeps all wavelengths focused on the slit.

As an option an additional, software-selectable, xenon flash lamp can be fitted to enable phosphorescence studies.

The Slits

The slits themselves are bilaterally, continuously adjustable from the computer in units of bandpass or millimeters. This preserves maximum resolution and instant reproducibility.

The Excitation Monochromator

The excitation monochromator is an aspheric design which ensures that the image of the light diffracted by the grating fits through the slit. The gratings themselves are plane, blazed gratings that avoid the two major disadvantages of the more common concave holographic gratings: poor polarization performance and inadequate imaging during scans that throws away light. The unique wavelength drive scans the grating at speeds as high as 80 nm/s. The grating grooves are blazed to provide maximum light in the UV and visible region.

The Reference Detector

Before the excitation light reaches the sample, a photodiode reference detector monitors the intensity as a function of time and wavelength. The photodiode detector has a wider wavelength response than the older, traditional rhodamine-B quantum counter, and requires no maintenance.

The Sample Chamber

A spacious sample chamber is provided to allow the use of a long list of accessories for special samples, and encourages the user to utilize a variety of sample schemes.

The Emission Monochromator

All the outstanding features of the excitation monochromator are also incorporated into the emission monochromator. Gratings are blazed to provide maximum throughput in the visible region.

The Emission Detector

Emission detector electronics employ photon-counting for the ultimate in low-light-level detection. Photon-counting concentrates on signals that originate from fluorescence emission, ignoring smaller signals originating in the detector tube (PMT). The more common method of analog detection (used by lower performance fluorometers) simply adds noise and signal together, masking weak emissions.

The emission detector housing also contains an integral high-voltage supply which is factory set to provide the signal-to-noise ratio.

Computer Control

The entire control of the FluoroMax originates in your PC with our FluorEssence™ software and is transmitted through a USB link. On start-up, the system automatically calibrates and presents itself for new experiments, or stored routines instantly called from memory.

Accessories

A wide variety of accessories are applications-oriented and most are interchangeable with the FluoroMax system.

 

FluorEssence Steady State and Frequency Domain Software

 

FluorEssence - Steady State and Frequency Domain Software

FluorEssence™ for Windows® sets a new standard for fluorescence software. FluorEssence™ merges spectrofluorometer data-acquisition directly to the famous processing power of Origin® software.

Simple clicks let you select a scan type, accessory or bring back a complete experimental set-up.

Revolutionize the Way You Operate Your Spectrofluorometer:

  • Simplified windows make data-acquisition intuitive even to the casual user.
  • With detector algebra, assemble input signals from different detectors into unique equations (including arithmetic or transcendental functions) that produce data in a format tailored to your application.
  • Select the special Real Time Control (RTC) window to optimize your run parameters before actually scanning.
  • Free video tutorials make FluorEssence™ even easier to learn.
  • Run a variety of accessories including polarizers, microwell-plate readers, temperature baths, autotitrators, phosphorimeters, and more.
  • Automatic correction for blanks and lamp output.
  • Change units for temperature, wavelength, and slit-width with the click of a mouse.

FluorEssence™ also includes the powerful processing and data-management tools of Origin® to streamline your laboratory’s operation.

Data-viewing, plotting, processing and management are all available, including extensive import and export facilities, view manipulation and creation, arithmetic applications, peak-picking and integration, data- management and much more!

 

Software Features

  • Data views in Workbook formats, keeping graphs, tables and notes together for each experiment
  • Zooming and scaling
  • Contour maps and profiles from 3D plots
  • Integration/DerivativeFit fluorescence data to Gaussian, Lorentzian, and custom curves
  • Standard arithmetic
  • 3D perspective
  • Smoothing
  • Deconvolute
  • Excitation/emission correction
  • Interpolation and extrapolation
  • Blank subtraction
  • Normalization
  • PLQY calculator wizard (for use with Quanta-Phi accessory)

 

DAS6 Decay Analysis Software

HORIBA Scientific decay analysis software recovers kinetic information (such as fluorescence lifetime and rotational correlation times) from luminescence decay data by fitting the raw decay data to one of a selection of kinetic models. Features such as shift iteration and reconvolution of instrument response function (IRF, or "lamp" profile) allow accurate recovery of multiple decay components even when the decay data is grossly distorted by the IRF.

DAS6 is designed to streamline the analysis of time-domain luminescence data, while still allowing for fine-tuning of parameters where necessary.  Analysis modules include reconvolution analysis of multi- exponential models plus analysis of more specialised fluorescence decay processes as detailed below.

Multi-Exponential

Analysis for up to 5 exponentials.

Batch Exponential

Batch mode supports datasets containing up to 10,000 decays and is ideal for applications where large amounts of lifetime data are generated e.g. lifetime imaging and reaction monitoring.  It features full reconvolution with up to five exponentials and shift iteration.

Global Exponential

Perform global analyses of up to 5 exponential components and 100 decay curves.

Distribution

Two methods are available for performing lifetime distribution analysis.  The first method employs a “Top Hat” distribution function which allows a single lifetime distribution to be fitted in conjunction with an additional discrete lifetime.  The second method is an optional module which will perform a Non-Extensive Decay distribution which allows up to five lifetime distributions to be modelled.

Exponential Series

Exponential series analysis of up to 30 terms.  This allows parameterisation of otherwise difficult data.

Foerster Energy Transfer

2D and 3D Foerster type energy transfer with an optional exponential component.

Yokoto-Tanimoto Energy Transfer

Yokoto-Tanimoto energy transfer taking into account diffusion with an optional exponential component.

Micellar quenching

Micellar quenching kinetics can help determine the surfactant aggregation number.

Exciplex

Exciplex analysis follows formation of an excited state species.

Anisotropy analysis

Impulse reconvolution of up to 2 correlation times and 5 fluorescence decay components.  Each model parameter, including shift, can be fixed at a pre-determined value or optimised as part of the parameter fitting.  The use of reconvolution allows accurate determination of decay times and rotational correlation times even when the observed decay is grossly distorted by the duration of the excitation pulse.

 

DataStation TCSPC Acquisition Software

DataStation is the latest measurement environment for time-domain fluorescence and is supplied with all HORIBA Scientific lifetime systems.

Lifetime systems are made up of various modules and components, including pulsed light sources, PMTs, monochromators, motorized polarizers, focusing optics, MCAs etc. DataStation integrates the control of all these components into one intuitive, mouse-driven Windows environment.

Lifetime systems can perform a variety of measurement functions, such as fluorescence decay measurement, phosphorescence decay measurement, time-resolved anisotropy measurements, steady-state spectra, time-resolved emission scan (TRES), temperature scans and more. These measurements are now routine using DataStation. Measurement templates are provided to quickly set up the instrument for the required measurement, and automated data acquisition is performed using computer controlled components.

Acquired data can be saved in a variety of formats including the .das format, HORIBA Jobin Yvon IBH v4.2, and ASCII, printed to your Windows printer, exported or copied to the clipboard for manipulation using a third-party application.

DataStation can be purchased separately for use on non-HORIBA Scientific systems, and we are happy to add custom functionality and interfacing (e.g. to additional monochromator types, motorized polarizer holders etc).

The DataStation software comes into its own when combined with a FluoroHub.

Functionality

DataStation evolves continually as new functionality is added in response to customer suggestion. The following is a partial list:

Graphical

  • Log or linear y-axis.
  • Auto scaling y-axis.
  • Multiple mouse zoom modes - x only, y only, x and y, pan, reset.
  • Reversed time-axis mode (automatically reverses data during reversed-TAC mode measurements).
  • Live display of cursor position, peak count, peak channel and total count.
  • Detachable and customizable toolbars with helpful hints.
  • Context-sensitive pop-up toolbars.
  • Intuitive tree navigation for files and hardware settings.
  • Tree navigation control window for FluoroHub control.
  • Real-time estimated average lifetime during acquisition.

 

TCSPC mode

  • Measurement presets - peak count or elapsed time.
  • Multiple traces on screen simultaneously.

TRES mode

  • Configure instrument for scan operation in just a few mouse clicks.
  • Scan excitation or emission monochromator.
  • Records 3D data (counts vs time vs wavelength) with live 3D progress display and interactive rotation.
  • Manual and automatic data slicing.

Anisotropy mode

  • Target difference count.
  • G-factor measurement.

MCA support

  • Canberra/Tennelec PCA3.
  • EG&G/Perkin Elmer Ortec Trump card.

Monochromator support

Polarizer support

  • HORIBA Scientific 5000U-02 polarizers.
  • PRA polarizers.

Temperature control support

  • HORIBA Jobin Yvon IBH dipstick temperature controller (as accessory to HORIBA Scientific 5000 series system only).
  • Cryostat (as accessory to HORIBA Scientific 5000 series system only).

System Requirements

The following PC specification are required to run DataStation. Measurement functionality depends on spectrometer hardware attached. Basic operation requires a supported MCA card or FluoroHub. Contact HORIBA Scientific for more information.

1 Gb RAM, 1 Gb HD space, DVD drive, 1024x768 graphics with minimum 256 color support, mouse or other pointing device. Windows™ XP or later (XP/Vista/7). Windows™ XP recommended.

 

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Aqualog® - Our compact, Benchtop Fluorometer for CDOM

 

Overview

Aqualog is the only instrument to simultaneously measure both absorbance spectra and fluorescence Excitation-Emission Matrices. EEMs are acquired up to a 100 times faster than with other instruments. Dedicated software automates traceable Quinine Sulfate Unit calibration and correction of inner-filter effects and Rayleigh and Raman scattering lines, enabling rapid export to modeling algorithms like PARAFAC.

multivariate analysis - AqualogAqualog now offers new deep UV absorbance and fluorescence excitation from 200 nm, in addition to a fully automated sample queue tool to facilitate continuous data acquisition, analysis and export for immediate multivariate analysis with the Solotmpackage from our partner, Eigenvector Research, Inc.

The NEW Aqualog® Datastream Dashboard facilitates completely automated analysis and reporting of a wide range of organic matter parameters that are critical for managing and optimizing the drinking water treatment process. The Datastream parameters have been selected to specifically target disinfection by-product issues, algal issues and other contamination components 

Features

Aqualog

Hardware

  • The only true simultaneous absorbance-fluorescence system available

  • TE-cooled CCD fluorescence emission detector for rapid data acquisition up to 100 times faster than any other benchtop fluorometer

  • Corrected UV-VIS absorbance detection path for stability and accuracy

  • Double grating excitation monochromator for superior stray light rejection

  • Matching bandpass for absorbance and fluorescence spectra

  • Automatic sample changer option (2 or 4 position)

  • Compatible with flow cells and titrator

Software

  • Optimized experiment set-up menus minimize user confi guration time

  • Complete NIST-traceable corrected fluorescence spectra automatcally generated

  • Spectral and kinetic analysis tools for both absorbance and fluorescence data

  • Methods and batch protocols for automating multiple sample measurement

New A-TEEM technology

A-TEEM technology uses absorbance, transmittance and EEM data to fingerprint molecules with high specificity and ultrahigh-sensitivity at a 6 million nm/min emission scan rate!

All colored molecules exhibit unique molecular absorbance and transmittance spectra; many colored molecules also exhibit unique fluorescence excitation and emission spectra that can be measured as an excitation-emission matrix (EEM). Simultaneously combining Absorbance–Transmission and EEM is a new technique (A-TEEM) that provides a distinct molecular fingerprint with numerous potential applications.

Aqualog combines an ultrafast CCD that’s up to 4,000 times faster than traditional PMT-based fluorometers, with our new A-TEEM technology so you can easily and effectively identify, quantify and understand individual organic compounds in complex mixtures in minutes. A-TEEM has already proven in many cases to be more effective in protein, vaccine, wine and water research, quality and process applications than HPLC and vibrational spectroscopy.

NEW Aqualog® Datastream Dashboard

Features

  • Seamless integration with Aqualog
  • Convenient HTML-based interface
  • Push-button method operation
  • Simple administrator level controls for calibration and method development

Benefits

  •  Easy access through internet or intranet
  •  Dashboard shows the latest readings, time series and tables for trends and analysis
  •  WTP can upload their own independent data

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Fluorolog Extreme

 

The first fully integrated supercontinuum white light laser-powered lifetime steady-state fluorometers.

The Fluorolog Extreme seamlessly combines our fluorometers with the high intensity and broad wavelength range of the supercontinuum lasers made by NKT Photonics.

What is a supercontinuum laser, also known as a white light laser? It is a very bright source that, unlike other lasers, simultaneously puts out light at a very wide spectral range from just above 400 nm to over 2 µm. Because of their brightness and broad spectral range, they make excellent sources for steady-state measurements. Furthermore, since the duration of the laser pulses is in the 10s of picoseconds range, these lasers can also be used as lifetime sources.

WHY GO EXTREME ?

  • Steady-State:  More excitation power for weak fluorescence signals, especially in the NIR.

  • It’s LIKE having A 1000W Xenon Lamp!

Features

  • Select any excitation wavelength from the visible to over 2 microns
  •   Complete optical, mechanical and software integration:
    • Kinematic adapter to mount excitation monochromator instead of xenon lamp
    • Control of laser power and wavelength directly from FluorEssence Software. Also, all laser parameters are storable in and recallable from XML method files, reports.

Specification

The world’s most sensitive fluorometer is made even more sensitive!

  • More excitation power, especially in the NIR, means more signal:

  • More excitation power, especially in the NIR

A Fluorolog/FluoroCube Extreme is comprised

  • Choice of any NKT SuperK Extreme model:

PLUS:

  • Any Fluorolog 3 (including existing units). Includes kinematic adapter to replace xenon lamp on entrance of excitation monochromator.

 

Dual-FL - The world's fastest Fluorometer

 

Overview

HORIBA Scientific combined the world’s only CCD based benchtop spectrofluorometer with a UV-VIS spectrophotometer and created the Dual-FL.

The Dual-FL features spectral rates as fast as 80,000 nm/s, and a water Raman S/N sensitivity greater than 20,000:1 RMS.

Collect incredibly rapid kinetics data, or complete excitation emission matrix (EEMs) in seconds.

And, it also has a built-in UV-VIS spectrophotometer. Use it as your lab UV-VIS, or collect simultaneous dual-mode fluorescence and absorbance data to correct inner-filter effects in your spectra.

Features

Hardware

  • The only true simultaneous absorbance-fluorescence system available

  • TE-cooled CCD fluorescence emission detector for rapid data acquisition up to 100 times faster than any other benchtop fluorometer

  • Corrected UV-VIS absorbance detection path for stability and accuracy

  • Double grating excitation monochromator for superior stray light rejection

  • Matching bandpass for absorbance and fluorescence spectra

  • Automatic sample changer option (two or four position)

  • Compatible with flow cells and titrator

Software

  • Optimized experiment set-up menus minimize user confi guration time

  • Complete NIST-traceable corrected fluorescence spectra automatcally generated

  • Spectral and kinetic analysis tools for both absorbance and fluorescence data

  • Methods and batch protocols for automating multiple sample measurement

Specification

Fluorescence Hardware

Light source

150W ozone free vertically mounted xenon arc lamp

Excitation range

200 - 800 nm (Dual-FL UV)

Excitation bandpass

5 nm

Excitation monochromator

Subtractive double monochromator

Excitation gratings

1200 gr/mm, 250 nm blaze

Excitation wavelength accuracy

±1 nm

Emission range

250 - 800 nm

Emission bandpass

5 nm

Emission spectrograph

Fixed, aberration-corrected 140 mm focal length

Emission grating

405 gr/mm; 250 nm blaze

Emission detector

TE-cooled back-illuminated CCD

Emission integration time

5 ms minimum

Hardware pixel binning

0.41, 0.82, 1.64, 3.28 nm/pixel

CCD gain options

4.5 e-/cts in medium gain, 9 e-/cts in low gain

Sensitivity

Water-Raman SNR > 20,000:1 (RMS method) (350 nm excitation, 30s integration)

Weight

33 kg (72 lbs)

Dimensions

LWH (618 x 435 x 336 mm); (24" x 17" x 13")

 

Absorbance Hardware

Scanning range

230 - 800 nm (optical)

Bandpass

5 nm

Slew speed

Max 500 nm/s

Optical system

Corrected single beam

 Detector

Si photodiode

Wavelength accuracy

±1 nm

Wavelength repeatability

+/- 0.5 nm

Photometric accuracy

±0.01 AU from 0 to 2A

Photometric stability

<0.002 AU per h

Photometric repeatability

 +/- 0.002 AU (0 to 1 AU)

Stray light

<1% measured with KI standard

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Deltaflex

 

Overview

The DeltaFlex TCSPC system is one of our Delta series of next generation TCSPC lifetime instrumentation. The DeltaFlex offers the ultimate in flexibility:

Firstly, this system is designed for the measurement of luminescence lifetimes ranging over 11 orders of magnitude, without the need to change cables or cards. The heart of the system is the DeltaHub timing electronics, which offers nearly lossless counting and allows for the measurement of lifetimes from 25ps to 1 second.  Comprised of our high repetition rate sources, high speed detectors and ultra-low deadtime electronics, the DeltaFlex enables fast and efficient acquisition of lifetime data.

Secondly, our new F-Link bus brings simplicity to modularity. Simply add any component to the instrument and the system automatically detects the new addition and makes it accessible from the software.

Finally, with biological probes and applications such as photovoltaics moving further into the NIR, capability for time-resolved measurements in this range is very important.  The DeltaFlex can couple with a range of NIR detectors to offer a solution.  

The DeltaFlex system uses our interchangeable range of DeltaDiode excitation sources, DeltaHub timing electronics and PPD picosecond detection modules.  Add excitation and/or emission monochromators for wavelength selection and complete spectral collection such as TRES. Our TDM-800 monochromator is specifically designed to give low temporal dispersion.  Systems are supplied with a sample holder equipped with a digital temperature sensor and magnetic stirrer as standard.

Accessories include a long list of DeltaDiode and SpectraLED sources, monochromators and peltier temperature control, to name a few.  The DeltaFlex can be easily upgraded to incorporate motorized devices through the F-Link spectrometer bus. 

DeltaFlex-NIR-H optical system shown with H10330 series detector and SpectraLED excitation source.

Features

  • Measure lifetimes from 25ps with laser diodes and PPD detection module
  • Up to 1,000 TCSPC measurements per second – ideal for kinetics studies
  • Monochromator wavelength selection  
  • DeltaDiodes available from UV to NIR.  Consult the DeltaDiode page for full details
  • SpectraLEDs can be used to acquire longer lifetimes (µs – s) and available from UV to NIR.
  • Upgrade to a monochromator based NIR system (DeltaFlex-NIR-H or DeltaFlex-NIR-R)
  • Easy to add accessories with F-Link bus
  • Comprehensive software package
  • Measurement modes
    • Lifetime – measure 25ps to 1s
    • Kinetic TCSPC – 1 to 10 000 decays measured sequentially in 1ms to 1min per decay
    • Anisotropy – reconvolution analysis to resolve shorter rotational correlation times
    • Time-Resolved Emission Spectra (TRES) – Collect up to 100 wavelength dependent decays
    • Steady-State

Specifications

DeltaFlex system

Minimum Lifetime

25ps with laser diode source*

Shortest measurement time

1 millisecond*

Repetition rates

10kHz - 100MHz with DeltaDiode, 0.1Hz - 10kHz with SpectraLED

Diode controller

DeltaDiode and SpectraLED

Prompt FWHM

<200ps FWHM with PPD/TBX and laser diode

Deadtime

10ns

Time ranges

10ns - 11s

Wavelength selection

  • Emission monochromator as standard 200-800nm.
  • 300-1200nm and 400-1600nm versions optional
  • Excitation and 2nd emission monchromators also available

Detector response

  • 250 - 650nm standard, 250 - 850nm and 300 – 900nm optional. 
  • NIR detectors to 1700nm available

PC interface

USB 2.0.  PC not included.  Requires Windows XP or Windows 7, 32/64-bit English language ver

System Footprint

75cm x 55cm nominal excluding PC

**With DeltaDiode and PPD attached, no cables

DeltaFlex-NIR upgraded systems

 

DeltaFlex

DeltaFlex-NIR-H

DeltaFlex-NIR-R

Measurable lifetime*

25ps to 1s

100ps to 1s

300ps to 1s

Interface

USB

USB

USB

Wavelength coverage of detector

250-650nm
(250-850nm or 300-900nm optional)

950-1400nm or 
950-1700nm

300-1400nm or 300-1700nm

* Dependent on sample and system configuration

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Download Datasheet

Nanolog® - Our modular Spectrofluorometer for Nanotechnology

 

Description

The Nanolog® series of spectrofluorometers are specifically designed for research in nanotechnology and the frontiers of nanomaterials. Based on the world-wide proven technology of the FluoroLog®, the NanoLog® detects fluorescence in the near-IR from 800 to 1700 nm (optional multi-channel detection to 2 µm, single-channel detection to 3 µm), with visible and UV options possible. With the NanoLog® comes specially designed software ideal for classifying SWNTs, performing energytransfer calculations, and saving custom routines and instrument layouts. A complete spectrum can be scanned as fast as a few milliseconds, and a full excitation-emission matrix scan can be taken in as little as seconds.

Nanolog - Perfect for quantum dots, too! 

Features

Features Rapid excitation-emission matrices in seconds High sensitivity in near-IR with InGaAs array High resolution Eases qualification and quantification of species and families of SWNTs Compatible with variety of detectors from UV to near-IR:Photomultiplier tube for highest sensitivity and timeresolved analysisPopular, cost-effective single-element InGaAsMulti-element CCD array for fast data-acquisition Resolve mixtures of quantum dots simultaneously Perform energy-transfer experiments Modular design for your ideal experimental setup.

 

Nanosizer® Software

Nanosizer® - for Single‐Walled Carbon Nanotube Excitation‐Emission Map Simulation and Analysis

Nanosizer® in Origin® Pro 8 simplifies the process for simulation and analysis for single‐walled carbon nanotube excitation‐emission map simulation and analysis. Nanosizer is used with our Nanolog spectrofluorometers, which are specifically designed for research in nanotechnology and nanomaterials. Nanosizer comes with our patented double-convolution-integral algorithm specially designed for determining chirality and diameter of single-walled carbon nanotubes.

Nanosizer® lets you simulate excitation-emission maps of SWNT near-IR fluorescence to compare to your actual data. Using built-in or custom libraries, Nanosizer® rapidly assigns specific peaks to particular SWNT (n,m) structures, and even generates helical maps. Nanosizer® also greatly simplifies FRET studies of SWNT bundles, length-distribution analyses, and nanotube purification analyses. Nanosizer®even offers a platform suitable to support future ISO and ASTM standards for identification and purification of semiconducting SWNTs.

Click here to download the Nanosizer brochure

Perfect for FRET in SWNT Bundles, Length Distribution Analysis, and Purification applications

Features and Benefits of Nanosizer® in OriginPro® 8

  • Efficient Region of Interest and Initial Model Parameterization
  • Virtually unlimited number of peaks
  • Global linking and fixing of peak parameters
  • Full constraints on all model peak parameters
  • Save Themes for rapid model parameterization
  • 2D analytical line shapes: Gaussian, Lorentzian and Voigt Convolution
  • Correct statistical weighting of residuals
  • Fully featured statistical analysis of fit peak parameters
  • Graphical and tabular presentation of fit results and residuals
  • Fits data in energy (cm–1, eV) or wavelength (nm) units
  • Compares peak parameters to user editable library for helix angle, diameter and (n,m) distribution plots and tables
  • Designed for ISO and ASTM Standards for Semiconducting SWNT Identification/Quantification

Specifications

  • 450 W intense broadband cw xenon lamp for bright excitation from UV to near-IR
  • Full excitation-emission matrices in seconds
  • Symphony II InGaAs array detectors: 800-1700 nm; 256 x 1,512 x 1, and 1024 x 1 pixel formats as small as 25 µm pitch; noise as low as 650 erms with liquid-nitrogen cooling for best signal-to-noise ratio; optional thermoelectric cooling; extended range option (1.1 - 2.2 µm)
  • iHR320 emission spectrograph: focal length = 320 mm; f/4.1; dispersion = 2.31 nm/mm; resolution = 0.06 nm (with slit); software-controlled triple-grating turret (all measured with 1200 grove/mm grating)
  • Solid-state near-IR detectors, photomultiplier tubes from UV to near-IR, time-correlated single-photon counting lifetime measurement (100 ps to 1 ms, UV to near-IR), phosphorimeter (1 µs to > 10 s, UV to near-IR), and frequency-domain lifetime measurement (10 ps to 10 µs, UV to near-IR) are also available

Schematics

Schematic of the NanoLog, showing the optical path from source through sample to detectors
 
 
 
 
 

DeltaPro

 

 

Overview

The DeltaPro has taken the complexity of time-correlated single photon counting (TCSPC) and made it simple and affordable so that any lab can exploit the power of fluorescence dynamics using TCSPC. This system enables the seamless measurement of luminescence lifetimes from picoseconds to 1 second, using our DeltaHub timing electronics.

The DeltaPro takes advantage of interchangeable pulsed laser-diode and LED light-sources. The NanoLED and DeltaDiode range of sources cover discrete emission wavelengths from 250 nm to the near-IR and enables measurement of lifetimes from ps to µs. By simply adding a SpectraLED source to the system, lifetimes of µs to 1 second can be measured. Lifetimes ranging over 11 orders of magnitude measured with one compact system.

The DeltaPro system is modular. The DeltaPro system can easily be upgraded to incorporate motorized devices, such as automated focusing and motorized polarizers through the F-Link spectrometer bus.

Features

  • Filter-based wavelength selection
  • Fast, reliable USB 2.0 interface ensures trouble-free data-transfer to laptop or desktop PC
  • Comprehensive analysis software
  • Detector range is 185 to 650 nm (Optional upgrade to 850 and 900nm)
  • Standard sample holder with magnetic stirrer and temperature sensor
  • Measure rotational correlation times using optional polarizers
  • Full range of DeltaDiode, NanoLED and SpectraLED diode light sources from UV to near-IR available as an option
  • Operation up to 100MHz with DeltaDiode light sources
  • F-Link spectrometer interface for plug-n-play upgrades
  • Measurement modes
    • Lifetime – measure 25ps to 1s
    • Kinetic TCSPC – 1 to 10 000 decays measured sequentially in 1ms to 1min per decay
    • Anisotropy – reconvolution analysis to resolve shorter rotational correlation times

Specifications

Minimum Lifetime

25ps with laser diode source*

Shortest measurement time

1 millisecond*

Repetition rates

10kHz - 1MHz with NanoLED, 10kHz - 100MHz with DeltaDiode, 0.1Hz - 10kHz with SpectraLED

Diode controller

DeltaPro-NL: NanoLED and SpectraLED.  
DeltaPro-DD: DeltaDiode and SpectraLED

Prompt FWHM

<200ps FWHM with PPD/TBX and laser diode

Deadtime

10ns

Time ranges

10ns - 11s (DeltaPro-DD)

Wavelength selection

Interchangeable filters (not included), monochromators optional

Detector response

250 - 650nm standard, 250 - 850nm and 300 - 900nm optional.

PC interface

USB 2.0.  PC not included.  Requires Windows XP or Windows 7, 32/64-bit English language ver.

System Footprint

75cm x 45cm x 25cm nominal excluding PC

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Download Datasheet 

Ultima™

 

Overview

Ultima - Our optimized fluorimeter for short lifetimes

Picoseconds to seconds: a compact fluorescence system optimized for short lifetime measurements

Ultima combines the latest in high temporal resolution TCSPC electronics, interchangeable high speed light sources and detector technologies, and our FluoroCube, the most flexible optical platform to offer the highest performance photon counting lifetime system available.

Choose from the most comprehensive range of pulsed light source and detector options, including:

  • DeltaDiode Picosecond laser diodes from 370 nm to NIR
  • Picosecond and Nanosecond LEDs from 260 nm to NIR
  • Ti-sapphire laser and multi-photon options
  • Highly integrated picosecond PMT modules
  • Microchannel plate PMTs for the ultimate in time resolution
  • UV to 1700 nm spectral coverage

Benefit from our 40 years of TCSPC experience by consulting with us to tailor the perfect lifetime system for your research.

Ultima-01(11)-DD

Lifetime system with emission monochromator (01) or excitation and emission monochromators (11) and diode laser excitation

  • Large sample compartment (compatible with cryostats)
  • Cell holder with facility for recirculating liquid temperature control
  • Motorized excitation optics with UV-grade fused silica lenses, filter holder and mount for polarizer*: a wide choice of interchangeable laser diode and LED-based excitation sources are available as accessories
  • Motorized emission optics with UV-grade fused silica lenses, filter holder and mount for polarizer*
  • Emission monochromator (01,11 models): TDM-800 Seya-Namioka type, featuring motorized wavelength drive (200 nm to 800 nm), integral safety shutters interlocked to lid of sample compartment, and synchronized adjustable slits (1 nm to 32 nm)
  • Excitation monochromator (11 model only): Seya-Namioka type, featuring motorized wavelength drive (200 nm to 800 nm), integral safety shutters interlocked to lid of sample compartment, and synchronized adjustable slits (1 nm to 32 nm)
  • DeltaDiode pulsed diode controller module (software controlled)**
  • PPD-650 (230-700nm) proprietary picosecond photon-detection module (interlocked with lid of sample compartment). PPD-850 and -900 available to extend spectral range to 850 and 920 nm accordingly
  • FluoroHub A+ timing electronics - measures picoseconds to seconds (depending on excitation source)
  • DataStation measurement software and DAS6 decay analysis software
  • All cables and interconnections
  • System supplied on a 37 x 70 cm baseplate

*Filters and polarizers are optional**DeltaDiodeTM sources must be ordered separately

Ultima Ultrafast 01-Ti

Our shortest lifetime (5 ps) system with emission monochromator, external mode locked laser (e.g.  Ti:sapphire)**, and MCP PMT detector.

  • Large sample compartment (compatible with cryostats)
  • Cell holder with facility for recirculating liquid temperature control
  • Motorized excitation optics with UV-grade fused silica lenses, filter and mount for polarizer*: a wide choice of interchangeable laser diode and LED-based excitation sources are available as accessories
  • Motorized emission optics with UV-grade fused silica lenses, filter and mount for polarizer*
  • Emission monochromator: TDM-800 Seya-Namioka type, featuring motorized wavelength drive (200 nm to 800 nm), integral safety shutters interlocked to lid of sample compartment, and synchronized adjustable slits (1 nm to 32 nm)
  • Laser input optics for use with external mode-locked laser**
  • R3809-50 MCP-PMT detector in cooled housing (interlocked with lid of sample compartment) with close-coupled CFD-2G-A picosecond timing amplifier/discriminator and Fluoro3PS ultra-stable HV detector power supply.
  • FluoroHub A+ timing electronics - measures picoseconds to seconds (depending on excitation source)
  • DataStation measurement software and DAS6 decay analysis software
  • All cables and interconnections
  • System supplied on a 37 x 70 cm baseplate

*Filters and polarizers are optional**Laser not included

Ultima Ultrafast 01-DD

Our short lifetime system with emission monochromator, interchangeable DeltaDiode laser excitation, and MCP PMT detector.

  • Large sample compartment (compatible with cryostats)
  • Cell holder with facility for recirculating liquid temperature control
  • Motorized excitation optics with UV-grade fused silica lenses, filter holder and mount for manual polarizer*: a wide choice of interchangeable laser diode and LED-based excitation sources are available as accessories
  • Motorized emission optics with UV-grade fused silica lenses, filter and manual mount for polarizer*
  • Emission monochromator: TDM-800 Seya-Namioka type, featuring motorized wavelength drive (200 nm to 800 nm), integral safety shutters interlocked to lid of sample compartment, and synchronized adjustable slits (1 nm to 32 nm)
  • DeltaDiode pulsed diode controller module (software controlled)**
  • R3809-50 MCP-PMT detector in cooled housing (interlocked with lid of sample compartment) with close-coupled CFD-2G-A picosecond timing amplifier/discriminator and Fluoro3PS ultra-stable HV detector power supply.
  • FluoroHub A+ timing electronics - measures picoseconds to seconds (depending on excitation source)
  • DataStation measurement software and DAS6 decay analysis software
  • All cables and interconnections
  • System supplied on a 37 x 70 cm baseplate

*Filters and motorized polarizers are optional**DeltaDiode sources must be ordered separately

Specification

Ultima system Specifications   

  • Minimum lifetime: 5ps (with femtosecond laser and MCP)
  • Measurement range: 5ns-22s
  • Electrical time resolution (time/pt): <400fs
  • Timing jitter: <10ps FWHM (<3ps RMS)
  • Maximum rep. rate: 20MHz (recommended)
  • Maximum histrogram size: 16k
  • Phosphorescence resolution: 42ns/pt
  • Interface: USB 2.0

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Ultima™ Extreme

 

Overview

The fully integrated supercontinuum white light laser-powered lifetime fluorometer.

In the Ultima Extreme, we seamlessly combine our fluorometer with the high intensity and broad wavelength range of a supercontinuum laser.

What is a supercontinuum laser, also known as a white light laser? It is a bright source that, unlike other lasers, light over a broad spectral range, from just above 400 nm to over 2 µm. Because of this range and pulse width characteristics, in the range of 10s of picoseconds or longer, it makes an excellent source option for TCSPC measurements.

WHY GO EXTREME ?

Lifetime: Allows you to choose any excitation wavelength.

It’s like having infinite Deltadiodes!

Features

  • Select any excitation wavelength from 400nm to over 2 µm for lifetime measurements with the 300-1200nm excitation monochromator or filters. Wavelengths below 430 nm can still be addressed using our existing DeltaDiodes, or optional doubler. 
  • Select pulsing frequency from 100 kHz – 20 MHz (using required pulse picker)
    • Complete optical, mechanical and software integration:
    • Adapter for use with excitation monochromator
    • Complete delay and signaling integration for synchronization
    • Control of wavelength, and pulse frequency directly from DataStation Software, in addition to monitoring the laser power.                     
  • Large sample compartment (compatible with cryostats)
  • Cell holder with facility for recirculating liquid temperature control
  • Motorized excitation optics with UV-grade fused silica lenses, filter and mount for polarizer*: a wide choice of interchangeable laser diode and LED-based excitation sources are available as accessories
  • Motorized emission optics with UV-grade fused silica lenses, filter and mount for polarizer*
  • Emission monochromator: TDM-1200 Seya-Namioka type, featuring motorized wavelength drive (300 nm to 1200 nm), integral safety shutters interlocked to lid of sample compartment, and synchronized adjustable slits (1 nm to 32 nm)
  • Emission monochromator: TDM-800 Seya-Namioka type, featuring motorized wavelength drive (200 nm to 800 nm), integral safety shutters interlocked to lid of sample compartment, and synchronized adjustable slits (1 nm to 32 nm)
  • R3809-50 MCP-PMT detector in cooled housing (interlocked with lid of sample compartment) with close-coupled CFD-2G-A picosecond timing amplifier/discriminator and Fluoro3PS ultra-stable HV detector power supply.
  • FluoroHub A+ timing electronics - measures picoseconds to seconds (depending on excitation source)
  • All cables and interconnections
  • System supplied on a 37 x 70 cm baseplate

DeltaTime

 

Overview 

The DeltaTime TCSPC lifetime plug-in offers acquisition speed, flexibility, and affordability unavailable in any other hybrid fluorescence solution available. DeltaTime seamlessly integrates monochromators, polarizers, and other accessories with the widest array of sources (LEDs, laser diodes, supercontinuum lasers) and detectors (including NIR), providing lifetime coverage from 25ps to 1sec over wavelengths spanning the UV to NIR.

The culmination of over 40 years of lifetime experience, DeltaTime highlights include: the fastest sources (up to 100 MHz), the widest lifetime ranges (ps to sec), virtually unlimited configurability and advanced lifetime analysis software. DeltaTime is part of our Delta series...truly the next generation of fluorescence lifetime systems.

Features

  • Fast…lifetime acquisition times from one millisecond
  • Sensitive…uses single-photon counting detection
  • Accurate…crystal locked timing circuits never require recalibration
  • Wide range…resolves lifetimes from 25ps to 1 second
  • Modular…easily reconfigured as measurement requirements evolve
  • Compact…desktop dimensions
  • Convenient…single USB 2.0 connection to PC

Specifications

 

DeltaPro-DD

DeltaPro-NL

Minimum lifetime

25 ps with laser-diode source

30 ps with laser-diode source

Shortest acquisition time

1 millisecond*

100 milliseconds*

Diode controller

DeltaDiode and SpectraLED

NanoLED and SpectraLED

Repetition rates

  • 10 kHz–100 MHz with DeltaDiode*
  • 0.1 Hz–10 kHz with SpectraLED
  • 10 kHz–1 MHz with NanoLED
  • 0.1 Hz–10 kHz with SpectraLED

Prompt FWHM

<200 ps FWHM with PPD and laser diode

Dead time

10 ns

Time ranges

10 ns – 11 s

100 ns–11 s

Wavelength selection

Interchangeable filters (filters purchased separately from HORIBA or others)

Detector response

250–650 nm standard; 250–850 nm and 300–900 nm optional

PC interface

USB 2.0. PC not included. Requires Windows® XP or Windows®  7, 32/64-bit English language ver.

System footprint

75 cm * 45 cm nominal excluding PC (depending on options)

Download Datasheet